Fig. 6.

LCA60 protected MERS-CoV–infected IFNAR-KO mice. We transferred 200 µL of diluted antibodies (15 mg/kg) in PBS into Ad5-hDPP4–transduced IFNAR-KO mice (6–12 wk) intraperitoneally 1 d after MERS-CoV infection. Mice were then infected with 1 × 10⁵ pfu MERS-CoV London1/2012 strain (A) or EMC/2012 strain (B–E) intranasally. Virus titers in the lungs were measured at the indicated time points. Additional groups of animals were monitored daily for morbidity, mortality, and body weight loss up to 14 d after infection (C). The histologic analysis was performed on animals killed on day 7 after infection (D and E). Mice were anesthetized and transcardially perfused with PBS followed by zinc formalin. Lungs were removed, fixed in zinc formalin, and paraffin embedded. Sections were stained with hematoxylin and eosin for histological analysis. (F) Intranasally delivered LCA60-protected MERS-CoV–infected mice. We transferred 50 µL of diluted antibody (10 mg/kg) in PBS intranasally into Ad5-hDPP4–transduced IFNAR-KO mice (6 wk, female) 1 d after infection with 1 × 10⁵ pfu of the EMC/2012 strain of MERS-CoV. Virus titers in the lungs were measured at the indicated time points. Titers are expressed as pfu/g tissue. n = 3 mice per group per time point. *P < 0.05 compared with hIgG1 or PBS groups.