Fig. S1.

Identification of RabGDIα as a Gbp2-interacting protein. (A) The sequences for RabGDIα and RabGDIβ are shown on the first and the second line, respectively. An asterisk indicates the identity of the amino acid sequence. The portion with the red square was recovered in the mass spectrometric analysis. (B and C) MEFs overexpressing RabGDIα (B) or RabGDIβ (C) untreated or treated with 10 ng/mL IFN-γ were lysed. The lysates were immunoprecipitated with anti-Flag and detected by protein immunoblot with the indicated antibodies. (D and E) Lysates of MEFs stably transfected with retroviral vectors encoding the Flag-tagged RabGDIα (D) or RabGDIβ (E) were subjected to Western blot using indicated Abs. Data are representative of two independent experiments (B–E).