Fig. S6.

NO production in macrophages and in vivo serum cytokine levels during the acute phase are normal in myeloid-specific RabGDIα-deficient mice. (A) Control and RabGDIα cKO macrophages were untreated or treated with TNF-α and/or IFN-γ for 24 h. The concentrations of NO^2- in the culture supernatant were measured. N.D., not detected. Indicated values are means ± SD of triplicates. (B) Sera were obtained from control or RabGDIα cKO mice (n = 4 per group) at the indicated time postinfection of 5 × 10³ T. gondii. Serum concentrations of the indicated cytokines were measured by ELISA. Data are mean ± SD of triplicates. (C) A standard curve of luciferase activity (x axis) and parasite number (y axis) was generated according to luciferase activities of serial dilutions of ME49-expressing luciferase (10⁴ to 10⁷). N.S., not significant. Data are representative of two independent experiments (A–C).