Fig. S9.

Generation of Gbp2-deficient MEFs. (A) The Cas9/gRNA-targeted site in the fourth exon of Gbp2 gene. (Top) Structure of the Gbp2 gene. Black and white boxes denote the coding and noncoding exons, respectively. (Middle) The target sequence for gRNA was labeled with green, and the PAM sequence was underlined. (Bottom) Sequences of the mutated allele contained 1 bp deletion and substitution of guanine to cytosine (KO1) or of a deletion of 2 bp (KO2) are shown. Both KO1 and KO2 cause a frameshift mutation. (B) IFN-γ–stimulated WT and Gbp2-deficient MEFs were lysed and detected with the indicated antibodies by Western blot. Data are representative of two independent experiments (B).