Table S1.
Primers used in this study
| Primer name | Enzyme | Sequence | Resulting plasmids and descriptions |
| RabGDIα_F | BamHI | 5′-GGATCCACCATGGATGAGGAATACGATGTGATTG-3′ | pRabGDIα_Flag |
| RabGDIα_R | XhoI | 5′-GCGGCCGCTCACTCGAGCTGATCAGCTTCTCCAAAGACATCA-3′ | pRabGDIα_Flag |
| RabGDIα_M132I_F | — | 5′-AAGATCTACAAAGTGCCATCCACCGAGACTGAGGCCTTGGCTTCTAATCTGATCGGCATG-3′ | pRabGDIα_Μ132Ι_Flag |
| RabGDIα_M132I_R | — | 5′-CGGTGGATGGCACTTTGTAGATCTTG-3′ | pRabGDIα_Μ132Ι_Flag |
| RabGDIα_Y39V_R | — | 5′-AAGAGCTCTCACCCCCAACGTAGGGGTTT-3′ | pRabGDIα_Y39V_Flag |
| RabGDIα_R218A/Y219A_F | — | 5′-AGCGAGTCCCTGGCCGCGGCTGGCAAGAGCCCCTAT-3′ | pRabGDIα_R218A/Y219A_Flag |
| RabGDIα_R218A/Y219A_R | — | 5′-ATAGGGGCTCTTGCCAGCCGCGGCCAGGGACTCGCT-3′ | pRabGDIα_R218A/Y219A_Flag |
| RabGDIα_E233S/R240A_F | — | 5′-GGCCTGGGTTCGCTGCCCCAGGGCTTTGCCGCATTGAGTGCC-3′ | pRabGDIα_E233S/R240A_Flag |
| RabGDIα_E233S/R240A_R | — | 5′-GGCACTCAATGCGGCAAAGCCCTGGGGCAGCGAACCCAGGCC-3′ | pRabGDIα_E233S/R240A_Flag |
| RabGDIβ_F | BamHI | 5′-GGATCCACCATGAATGAGGAATACGACGTGATCGTGCTGGGC-3′ | pRabGDIβ_Flag |
| RabGDIβ_R | XhoI | 5′-GCGGCCGCTTACTCGAGGTCTTCTCCATAAATGTCATTCTTCTTGCGC-3′ | pRabGDIβ_Flag |
| Gbp1_F | BamHI | 5′-GGATCCACCATGGCCTCAGAAATCCACATGAAAG-3′ | pHA_Gbp1 |
| Gbp1_R | NotI | 5′-GCGGCCGCTTAAAGTATGGTGCATGATCGAGGTGG-3′ | pHA_Gbp1 |
| Gbp2_F | BglII | 5′-AGATCTACCATGGCCTCAGAGATCCACATGTCG-3′ | pHA_Gbp2 |
| Gbp2_R | NotI | 5′-GCGGCCGCTCAGAGTATAGTGCACTTCCCAGACG-3′ | pHA_Gbp2 |
| Gbp2_C586S_R | NotI | 5′-GCGGCCGCTCAGAGTATAGTGCTCTTCCCAGACG-3′ | pHA_Gbp2_C586S |
| Gbp2_K51A_R | — | 5′-CAGCTAGCTTGTTCATCAGGTAGGATGCGCCTGTGCGGTAGAGGCCCACG-3′ | pHA_Gbp2_K51A |
| Gbp3_F | BamHI | 5′-GGATCCACCATGGAGGCACCCATTTGTCTGGTGGAA-3′ | pHA_Gbp3 |
| Gbp3_R | NotI | 5′-GCGGCCGCCTAACTACTTAGTGAGCCGAGG-3′ | pHA_Gbp3 |
| Gbp5_F1 | EcoRI | 5′-GAATTCACCATGGCCCCAGAGATTCACATGCC-3′ | pHA_Gbp5 |
| Gbp5_R1 | — | 5′-CATCTGATGTGATGGCATGCCCATTGG-3′ | pHA_Gbp5 |
| Gbp5_F2 | — | 5′-CCAATGGGCATGCCATCACATCAGATG-3′ | pHA_Gbp5 |
| Gbp5_R2 | NotI | 5′-GCGGCCGCTTAGCTTATAACACAGTCATGATG-3′ | pHA_Gbp5 |
| Gbp7_F | BamHI | 5′-GGATCCACCATGGCATCTGGTCCCAACATGGAG-3′ | pHA_Gbp7 |
| Gbp7_R | NotI | 5′-GCGGCCGCTTAGAGTTTTCTAACTTTGTCTGAA-3′ | pHA_Gbp7 |
| Rab10_F | EcoRI | 5′-GAATTCATGGCGAAGAAGACGTACGACCTG-3′ | pHA_Rab10 |
| Rab10_R | NotI | 5′-GCGGCCGCTCAGCAGCACTTGCTCTTCCAGCC -3′ | pHA_Rab10 |
| RabGDIαKO_SA_F | SacII | 5′-CCGCGGCTTCGTGTCACTCATTTCTGCACTG-3′ | pKO-RabGDIα |
| RabGDIαKO_SA_R | SalI | 5′-GTCGACGTACTAGAGTGCAGGAACACAGGAC-3′ | pKO-RabGDIα |
| RabGDIαKO_MA_F | XhoI | 5′-CTCGAGTCCTGTGTTCCTGCACTCTAGTAC-3′ | pKO-RabGDIα |
| RabGDIαKO_MA_R | BamHI | 5′-GGATCCGTAAGGGTCCTCTAGGACTACACAG-3′ | pKO-RabGDIα |
| RabGDIαKO_LA_F | MluI | 5′-ACGCGTCTGTGTAGTCCTAGAGGACCCTTAC-3′ | pKO-RabGDIα |
| RabGDIαKO_LA_R | NotI | 5′-GCGGCCGCGATATGCAAGTTGTGCACACCCACC-3′ | pKO-RabGDIα |
| RabGDIα_screening_F | — | 5′-ATGCAGAGTCCGGTATCCGATCCAT-3′ | PCR screening |
| RabGDIα_WT_R | — | 5′-CACCTCGGTGATAGAGATAGGGAAG-3′ | PCR screening |
| RabGDIα_KO_R | — | 5′-TACGTGCCAGCCATCTTAGAGATACAG-3′ | PCR screening |
| RabGDIβ_WT_F | — | 5′-GATGCCATTGCTGATACCTGGACTG-3′ | PCR screening |
| RabGDIβ_WT_R | — | 5′-GAGTGAAATACCACTCTGATGGCTGG-3′ | PCR screening |
| RabGDIβ_KO_F | — | 5′-CTGTCGCTGTCTTCAAATGCACCAG-3′ | PCR screening |
| RabGDIβ_KO_R | — | 5′-GCCTGGCGCACTATTCAATACCATC-3′ | PCR screening |
| RabGDIα_qpF | — | 5′-GCTGATCTGTGATCCCAGTTACATCCC-3′ | Quantitative RT-PCR |
| RabGDIα_qpR | — | 5′-TTCTGCAGTCTCTACAGTGGTGCTGG-3′ | Quantitative RT-PCR |
| Gdi2_gRNA1_F | — | 5′-TTTCTTGGCTTTATATATCTTGTGGAAAGGACGAAACACCgcttttctaagcattcacta-3′ | Gdi2_gRNA1 |
| Gdi2_gRNA1_R | — | 5′-GACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACtagtgaatgcttagaaaagc-3′ | Gdi2_gRNA1 |
| Gdi2_gRNA2_F | — | 5′-TTTCTTGGCTTTATATATCTTGTGGAAAGGACGAAACACCgtttgacataaacacgcccc-3′ | Gdi2_gRNA2 |
| Gdi2_gRNA2_R | — | 5′-GACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACggggcgtgtttatgtcaaac-3′ | Gdi2_gRNA2 |
| Gbp2_gRNA1_F | — | 5′-TTTCTTGGCTTTATATATCTTGTGGAAAGGACGAAACACCgtcatcacctgaccagagtg-3′ | Gbp2_gRNA1 |
| Gbp2_gRNA1_R | — | 5′-GACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACcactctggtcaggtgatgac-3′ | Gbp2_gRNA1 |
| Gdi2_T7gRNA1_F | — | 5′-TTAATACGACTCACTATAGGgcttttctaagcattcactaGTTT-3′ | Gdi2_gRNA1 |
| Gdi2_T7gRNA2_F | — | 5′-TTAATACGACTCACTATAGGgtttgacataaacacgccccGTTT -3′ | Gdi2_gRNA2 |
| Gbp2_T7gRNA2_F | — | 5′-TTAATACGACTCACTATAGGgtcatcacctgaccagagtgGTTT-3′ | Gbp2_gRNA1 |
| gRNA_common_R | — | 5′-AAAAGCACCGACTCGGTGCCACTTTT-3′ | Gdi2_gRNA1, Gdi2_gRNA2, Gbp2_gRNA1 |
| T7Cas9_IVT_F | — | 5′-taatacgactcactatagggagaATGGACAAGAAGTACTCCATT-3′ | Cas9 mRNA |
| Cas9_R | — | 5′-ggatccGCTATGGCAGGGCCTGCCGCCCCGA -3′ | Cas9 mRNA |
| Gbp2_indel_F | — | 5′-catagggatggcaccacatgttgtg-3′ | Screening the mutation of the Gbp2 locus |
| Gbp2_indel_R | — | 5′-aggagtccaagtgattctgctaagc-3′ | Screening the mutation of the Gbp2 locus |
—, no restriction enzyme sites in primers.