Fig. 4.

Summary of AFL1 interactions and localization. AFL1 was found to be a peripheral membrane protein associated with both PM and endomembrane. At the PM, AFL1 interaction with AP2-2a and colocalization with CLC indicate a role in vesicle formation. Structural predictions also suggest AFL1 may interact with actin microfilaments. In endomembranes, AFL1 interacts with PDI5 and NAI2, which are negative effectors of drought response. In both membranes, AFL1 can also potentially interact with itself to form higher molecular weight complexes. Together, these roles of AFL1 have dramatic effect on drought response.