Table S2.
Oligonucleotides used in this study
| Name | Sequence (5′→3′) | Description |
| Primers for making mutant constructs | ||
| BBC125 | CCATAATCGCCAAGGTGTTTTG | ΔideA or ΔIdeA::KanR F1 (up arm) |
| BBC131 | gtcgacggatccccggaatATTCATGTAAATTGGCCAAAAATGAC | ΔideA::KanR deletion R1 (up arm) |
| BBC132 | gaagcagctccagcctacaTGCTAGCATGTATCCACAACCTAAC | ΔideA::KanR deletion F2 (down arm) |
| BBC129 | GTTAGGTTGTGGATACATGCTAGCAATTCATGTAAATTGGCCAAAAATGAC | ΔideA R1 (up arm) |
| BBC130 | GTCATTTTTGGCCAATTTACATGAATTGCTAGCATGTATCCACAACCTAAC | ΔideA F2 (down arm) |
| BBC126 | GGCTTTGAGCAAGATATCCAGTATG | ΔideA or ΔideA::KanR R2 (down arm) |
| ABD123 | ATTCCGGGGATCCGTCGAC | KanR cassette for ΔIdeA::KanR F (middle) |
| ABD124 | TGTAGGCTGGAGCTGCTTC | KanR cassette for ΔIdeA::KanR R (middle) |
| ABD820 | CGCTCTTATCTGCTTGGATAATGG | ΔdprA F1 (up arm) |
| ABD998 | gtcgacggatccccggaatCATTAACTGGCATCATCAACC | ΔdprA R1 (up arm) |
| ABD999 | gaagcagctccagcctacaTAGCTATGATGATGGATATTTTGATG | ΔdprA F2 (down arm) |
| ABD823 | TGAAGTACAAGGCCAGTTACTGG | ΔdprA R2 (down arm) |
| prm361 | attccggggatccgtcgacCTGCAGTTCagaagcagctccagcctaca | ΔdprA mini-FRT F (middle) |
| Prm362 | tgtaggctggagctgcttctGAACTGCAGgtcgacggatccccggaat | ΔdprA mini-FRT R (middle) |
| Primers for confirming mutant strains | ||
| BBC122 | ATGGTAAGCCGCTTGACTGG | ICE status F |
| BBC124 | CAGTAGGAACAAGGCCTACAGC | ICE integrated (used with BBC122 = 702 bp product if ICE is integrated in prfC) R |
| BBC123 | GCACGTCCGAAAAGCAGGAC | ICE absent (used with BBC122 = 523 bp product if ICE is not integrated in prfC) R |
| BBC120 | CGAAGGGGCTCTTCATCTTGC | ideA status F |
| BBC121 | TCTGGGCGAGACTCAATACATC | ideA status R (used with BBC120 = 862 bp product if IdeA is present and 190 bp if strain has an in-frame deletion in IdeA) |
| ABD725 | GAAGCAGCTCCAGCCTACA | dprA status F |
| BBC190 | AACAGCAGGAAACCACGCG | dprA status (used with ABD725 = 280 bp product if the strain has an in-frame deletion in dprA) R |