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. 2015 Jul 27;112(33):10443–10448. doi: 10.1073/pnas.1513341112

Fig. S4.

Fig. S4.

Induction of endogenous IFN-λs and IFN-β in HCV-infected primary human hepatocytes and Huh-7–TLR3 cells. (A) Primary human hepatocytes were infected with JFH1 HCVcc (MOI = 2) and harvested 5 d later. The expression of IFN-β, IFN-λ1, and IFN-λ2 was examined at the mRNA level by TaqMan real-time quantitative PCR and at the protein level by ELISA. (B) Huh-7–TLR3 cells were infected with JFH1 HCVcc (MOI = 10). The infected cells were harvested at the indicated time points, and the expression of IFN-β, IFN-λ1, and IFN-λ2 was examined at the mRNA level by TaqMan real-time quantitative PCR and at the protein level by ELISA. The data represent the means ± SEM (n = 3). *P < 0.05, **P < 0.01 compared with control.