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Differential responses to exogenous IFN-α, IFN-β, and IFN-λ1 in IFN-λ3–pretreated cells. Huh-7.5 cells were pretreated with IFN-λ3 for 5 d, and treated with IFN-α, IFN-β, and IFN-λ1. Immunoblotting was performed 30 min after IFN stimulation to evaluate the phosphorylation of STAT1 (A), and real-time qPCR was performed 6 h after IFN stimulation to measure the level of Mx1 mRNA (B). Bar graphs represent the means ± SEM (n = 3). *P < 0.5.
