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. 2015 Aug 3;112(33):10509–10514. doi: 10.1073/pnas.1506486112

Fig. 5.

Fig. 5.

Astrocyte expression of Rac1 or Mfge8 dominant negative transgenes inhibit discrete aspects of myelin phagocytosis. (A) Percentage of debris myelin enwrapped by lamellar processes at stage 62 per ON. (B) Percentage of axon area that represents focal myelin detachments per axon at stage 66, per ON. (C) Myelin/axoplasm ratio per axon per ON at stage 66. (D) Percentage of debris myelin by area per ON at stage 66. (E) Mean size of individual debris myelin units per ON at stage 66. (F) Immunofluorescence due to Tg(Blbp:Mfge8-mCherry) transgene or Tg(Blbp:Mfge8D91EmCherry) transgene in ON cross-sections at stage 66. (Scale bar: 50 μm.) (G) mCherry signal per unit area is elevated in the Mfge8D91E-mCherry transgene relative to the Mfge8-mCherry transgene at all stages. In A–D, measurements are from traced TEM micrographs. Mean ± SD is shown (n = 16 control and n = 8 transgenic in A, D, and E; n = 12 control and n = 8 transgenic in B and C). *P < 0.05; ***P < 0.001 [by Dunnett’s test (A, D, and E) or the Games–Howell test (B, C, and G)].