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. 2015 Aug 3;112(33):E4565–E4570. doi: 10.1073/pnas.1505356112

Fig. 3.

Fig. 3.

Rrm3 limits the frequency of fork rotation and precatenation at stable protein–DNA sites, whereas Tof1 acts more generally. Cells deleted for RRM3 and containing the top2-4 allele and (A) plasmid pRS426 or (B) plasmid tRNApRS316 and cells deleted for TOF1 and containing the top2-4 allele and (C) plasmid pRS426 or (D) plasmid tRNApRS316 were cultured and collected, DNA was prepared, and DNA catenation analysis was carried out as described in Fig. 1. Error bars or values are average deviation.