Fig. S8.

(A) Viability assay of wild-type, top2-td, tof1Δ, tof1Δ top2-td, mrc1Δ, and mrc1Δ top2-td cells under the permissive condition of growth on YPD at 25 °C or conditions of partial transcriptional repression of TOP2 (+12.5 µg/mL doxycycline). (B) Partial depletion of Top2 activity does not lead to an increase in “cut” mitosis in mrc1Δ cells. top2-td, mrc1Δ, and mrc1Δ top2-td cells grown under semirestrictive conditions (YPD, 37 °C + doxycycline) following synchronous release from G1 were analyzed for cytological analysis for cut and divided nuclei and chromosomal aneuploidy by cellular DNA content using FACS analysis (Bottom).