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. 2015 Sep;94(9):1276–1285. doi: 10.1177/0022034515592581

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© International & American Associations for Dental Research 2015

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Figure 4. — Soft diet (SD) does not rescue periodontal phenotype in Bsp^-/- mice. (A) Micro–computed tomography images demonstrate progressive tooth root resorption (green arrows) and alveolar bone (AB) loss (red arrows) in both Bsp^-/- diet groups at 8 and 20 wk. HD, hard diet. (B, D, F, H) Hematoxylin and eosin–stained first molar sections in 8-wk-old mice reveal that Bsp^-/- mice in both diet groups feature identical phenotypes, marked by lack of acellular cementum (white arrowhead), periodontal ligament (PDL) detachment (*), and epithelial (EP) downgrowth (yellow arrows); dentin (DE). (C, E, G, I) Picrosirius red–stained sections under polarized light microscopy demonstrate loss of PDL collagen organization in both Bsp^-/- diet groups.