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. 2015 Sep;94(9):1233–1242. doi: 10.1177/0022034515593465

Figure 4.

Figure 4.

NLRP3, apoptosis-associated speck-like protein containing CARD (ASC), and caspase 1 are dispensable for tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6), which are secreted in a canonical pathway. (A, B) Tnfa and Il6 mRNA expression in bone marrow–derived macrophages (BMDMs) from wild-type (WT; C57BL/6), NLRP3-/-, ASC-/-, or Casp1-/- stimulated by Porphyromonas gingivalis strains 381 or DPG3 at a multiplicity of infection of 100 for 3 h. Total RNA from each group was extracted, reverse transcribed, and quantified by quantitative reverse transcription polymerase chain reaction. (C, D) TNFα and IL-6 secretion was analyzed by ELISA in supernatants from BMDMs from WT (C57BL/6), NLRP3-/-, ASC-/-, or Casp1-/-. Culture supernatants were collected after 6 h of infection with P. gingivalis strains 381 or DPG3 (multiplicity of infection of 100). Data are shown as mean ± SD and are representative of 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.