Figure 4.

Measuring densities and mislabeling probability by applying SpIDA to a single oligomer population. Twenty-five simulated images of varying size containing 10 tetramers/BA were generated to measure the accuracy of one-population SpIDA in the presence of mislabeling (Eq. 5). The set density (solid line), SpIDA recovered densities (A), set labeling percentage p = 80%, and measured p value (B) are presented as a function of the size of the image. The error bars correspond to SDs. Example images where all subunits are labeled (p = 100%) (C), and then mislabeled (p = 80%) (D), with the corresponding intensity histograms (E). The image size was 300 × 300 pixels.