Figure 8.

Nuclei in LMNA^−/− MEFs flatten faster than WT cells. Nuclei in WT MEFs are less flattened at 1 h after cell seeding (A) compared with LMNA^−/− cells that have extremely flat nuclei. The degree of cell seeding is small in WT at 1 h and increases by 6 h; LMNA^−/− cells, however, are well spread at 1 h. Scale bar in the x-y view is 20 μm and in the x-z view it is 10 μm. The lack of cell spreading, higher nuclear heights and lower nuclear widths at 1 h in WT cells compared with LMNA^−/− cells is evident in the scatter plots of nuclear aspect ratio versus cell spreading area (B), as well in the average values of nuclear aspect ratio and spreading area (C) and (D) (^∗p < 0.05; n$\ge$ 43; all comparisons are with WT cells at 1 h spreading). All data are shown as mean $\pm$ SEM. To see this figure in color, go online.