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(a) Design of the dhps splice-blocking morpholino (MO). (b) Injection of the dhps MO results in a loss of exon 2, as confirmed by RT-PCR from control and MO-injected Tg(ptf1a:gfp) embryos; wildtype dhps, 385 bp; E2 deletion in dhps, 172 bp. (c) dhps MO-injected Tg(ptf1a:gfp) embryos at 72 hpf show a significant pancreatic phenotype after low dose MO injection (1 ng or 2 ng). E, exon; bp, base pair.
