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. 2015 Aug 18;17(7):598–611. doi: 10.1016/j.neo.2015.07.006

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Dose-dependent activation of β-catenin transcriptional activity in CP-A cells via Wnt3a: (A) Luciferase assay in CP-A cells following treatment with different concentrations of rhWnt3a for 24 hours. Lithium chloride served as positive control (*P < .05; **P < .01, t test). (B) Western blot analysis of total β-catenin and AXIN2 protein expression in CP-A cells following treatment with 200 ng/ml of rhWnt3a for 24 hours. GAPDH served as a loading control. (C) Immunofluorescence staining for total β-catenin in CP-A cells following rhWnt3a treatment as described above verified nuclear localization of β-catenin in CP-A cells (arrows).