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. 2015 Aug 18;17(7):598–611. doi: 10.1016/j.neo.2015.07.006

Figure 6.

Figure 6

Dkk1 suppresses Wnt3a-mediated β-catenin transcriptional activation in CP-A cells: (A) Luciferase assay (top) and qRT-PCR analysis of AXIN2 gene expression (bottom) in CP-A cells following stimulation for 24 hours with rhWnt3a (200 ng/ml) and rhDkk-1 (500 ng/ml) for 24 hours as indicated. (B) Luciferase assay (top) and qRT-PCR analysis of Dkk1 gene expression (bottom) in CP-A cells following siRNA-mediated Dkk1 gene silencing. (C) qRT-PCR analysis of AXIN2 gene expression in CP-A cells following siRNA-mediated Dkk1 gene silencing and stimulation for 24 hours with rhWnt3a (200 ng/ml), as indicated. The histograms demonstrate mean AXIN2 gene expression (normalized to β-actin) and TOP flash relative to unstimulated cells of three independent experiments (*P < .05; t test).