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. 2015 Aug 18;17(7):598–611. doi: 10.1016/j.neo.2015.07.006

Table 1.

Sequence of Primers Used for RT-PCR Analysis

Gene Name Official Symbol Gene ID Sequence
β-actin ACTB NM_001101.3 Forward: 5'-CAC TCT TCC AGC CTT CCT TC-3'
Reverse: 5'-GGT GTA ACG CAA CTA AGT CAT AG-3'
Cyclin D1 CCND1 NM_053056.2 Forward: 5'-CAT CTA CAC CGA CAA CTC CAT C-3'
Reverse: 5'-TCT GGC ATT TTG GAG AGG AGG-3'
Axin2 AXIN2 NM_004655.3 Forward: 5'-TAC CGG AGG ATG CTG AAG GC-3’
Reverse: 5'-CCA CTG GCC GAT TCT TCC TT-3'
Dickkopf-1 DKK1 NM_012242.2 Forward: 5'-AGC GTT GTT ACT GTG GAG AAG-3'
Reverse: 5'-GTG TGA AGC CTA GAA GAA TTA CTG-3'
c-MYC MYC NM_002467 Forward: 5’-ACC AGA GAA ACC TAA CAG TGC-3’
Reverse: 5’-CTC TTT CAT TTC GGC CAG TTC-3’
Wnt3a WNT3A NM_033131 Forward: 5'-CCA TCC TCT GCC TCA AAT TC-3’
Reverse: 5’-TGG ACA GTG GAT ATA GCA GCA-3’
CDX-2 CDX2 NM_001265 Forward: 5’-GGA GCT GGA GAA GGA GTT TC-3’
Reverse: 5’-TTT CCT CTC CTT TGC TCT GC-3’
SOX-9 SOX9 NM_000346 Forward: 5’-GAC CAG TAC CCG CAC TT-3’
Reverse: 5’-TTC ACC GAC TTC CTC CG-3’
BMP-4 BMP4 NM_001202 Forward: 5’-TTG AGT ATC CTG AGC GCC CG-3’
Reverse: 5’-GTT TAT ACG GTG GAA GCC CC-3’