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. 2015 Aug 24;6:866. doi: 10.3389/fmicb.2015.00866

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Copyright © 2015 Kaur, Pethani, Kumar, Kim, Sunna, Kautto, Penesyan, Paulsen and Nevalainen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal laser scanning microscope (CLSM) images of interactions between P. aeruginosa (PAO1, PASS1, and PASS2) and S. aurantiacum (WM 06.482 and WM 08.202) as observed after co-incubating both the organisms in SCFM liquid medium at 37°C for 24 h. P. aeruginosa cells are stained with Syto9 (shown in green) and S. aurantiacum with Mito-tracker deep red FM (shown in blue). 3D re-construction of CLSM datasets was performed using IMARIS software package (Bitplane). Scale bar = 50 μm. (A–C) CLSM images of co-culture of WM 06.482 with PAO1, PASS1, and PASS2, respectively. (D–F) CLSM images of co-culture of WM 08.202 with PAO1, PASS1, and PASS2, respectively.