Table 2.

Sequence of primers used for the construction of transformation cassettes.

Primer name	Sequence (5′–3′)
mCherry. fwd	GAA GAACCT CTT AAC CTC TAG (pki sequence) ATG GTG AGC AAG GGC GAG G
mCherry. rev	CAT GCG GGT ACC (KpnI) CTA TTA CTT GTA CAG CTC GTC CAT GC
pki. fwd	TGC TGC GAT ATC (EcoRV) CTT AAG TTA G TA ACT AGT GGA TC
pki.rev	CTC GCC CTT GCT CAC CAT (mCherry sequence) CTA GAG GTT AAG AGG TTC TTC
pki-hph. fwd	TAC GCG GCG CGC CCT TAA G (AflII) TT AG T AAC TAG TGG ATC
pki-hph.rev	CAT GCT AAG CTT (HindIII) CTA TTC CTT TGC CCG CGG AC

The primers contain engineered restriction sites shown in shading and the overlapping sequences are shown in bold.