Table II.

Steady-state kinetic parameters of wild type (WT) ALAS and circularly $K_m^{\mathrm{S}-\text{CoA}}$ permuted ALAS variants

Protein	$K_d^{\text{Gly}}$	kcat	$K_m^{\text{Gly}}$	$K_m^{\mathrm{S}-\text{CoA}}$	$k_{\text{cat}}/K_m^{\text{Gly}}$	$k_{\text{cat}}/K_m^{\mathrm{S}-\text{CoA}}$
	mM	s–1	mM	µM	s–1 · mM–1	s–1 · µM–1
WT ALAS	29.7 ± 0.8	0.016 ± 0.002	14.0 ± 2.0	11.0 ± 1.0	0.0011	0.0015
L25	32.0 ± 5.0	0.016 ± 0.003	10.7 ± 0.5	2.6 ± 0.4	0.0015	0.0062
Q69	67.0 ± 3.0
N404	11.3 ± 0.2	0.057 ± 0.003	5.1 ± 0.2	21.0 ± 2.0	0.0112	0.0027
N408	40.0 ± 4.0	0.033 ± 0.005	19.0 ± 3.0	8.1 ± 0.3	0.0017	0.0041