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Patch-clamp recording of neuronal somata during axon stretch. (A) Patch-clamp head stage positioning within an elongated well of a customized stretch-grown bioreactor. (B) Overview of head-stage, linear motion table, and stretch-growth bioreactor fixed to a customized microscope stage. (C) Orientation of somata, growth cones, and patch electrode within bioreactor. (D) Patch clamp of a stretch-grown cell, bar = 25 μm.
