Table 7.13.1.
Fitting parameters for data presented in Figures 7.13.8 and 7.13.9 when fitted with genetic algorithm-Monte Carlo analysis to a reversible self-association model for a monomer-dimer systema
| Parameter | Measured value | 95% Confidence interval | Target value |
|---|---|---|---|
| Monomer molecular weight | 19.76 kDa | 18.46 kDa, 21.06 kDa | 20 kDa |
| Monomer frictional ratio | 1.23 | 1.19, 1.27 | 1.25 |
| Dimer frictional ratio | 1.21 | 1.17, 1.25 | 1.25 |
| Association constant | 0.877 | 0.622, 1.133 | 1.0 |
| koff rate | 0.00228/sec | 0.00034/sec, 0.0042/sec | 0.001/sec |
a
Unlike in an SE experiment, the SV experiment not only provides a reliable equilibrium constant, but also provides kinetic information, as well as shape information for all species. These data were simulated with realistic noise, and the variance is reflected in the 95% confidence intervals derived from the Monte Carlo analysis.