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. 1994 Dec 20;91(26):12549–12553. doi: 10.1073/pnas.91.26.12549

Genetic selection of growth-inhibitory sequences in mammalian cells.

D G Pestov 1, L F Lau 1
PMCID: PMC45476  PMID: 7809075

Abstract

To assess the role of mitogenically activated genes in the control of cell proliferation, we have taken a genetic approach based on the premise that blocking the function of an essential gene should lead to growth inhibition. Using a newly developed selection procedure, we isolated growth-inhibitory sequences from a pool of random cDNA fragments of 19 growth-related genes associated with the G0/G1 transition. These sequences encode potential dominant negative variants of c-Fos, JunB, and p44MAPK that may interfere with their growth-related functions. We anticipate that this procedure, which allows for the selection of sequences that cause a growth-inhibition phenotype, may have broad applications in the identification and analysis of genes that regulate cell growth.

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