University of Wisconsin	National Institute of Aging
Rhesus monkeys (n)	76 (46 m, 30 f)	120 (60 m, 60 f)
Genetic origin of monkeys	India	China & India
Age at baseline	All adult	Juvenile (20 m, 20 f) Adolescent (20 m, 20 f) Old (20 m, 20 f)
Housing	Single caged	Single caged
Randomization	1 CR: 1 Control	1 CR: 1 Control
Dietary regimen of CR monkeys	30% restriction from a BL intake assessed individually	30% restriction from BL intake levels based on NRC guidelines
Dietary regimen of control monkeys	Fed ad libitum	Controlled allotment of food each day to avoid obesity (~5-10% CR)
Meal patterns	Morning meal, plus 100 Kcal integration of food at late afternoon	Twice a day
Source of nutrients	Semipurified diet rich in refined foods	Natural ingredients (Pesco-vegetarian diet)
Macronutrient Composition of diets	15% protein (lactalbumin) 10% fat (corn oil) 65% carb (cornstarch, sucrose) 28.5% from sucrose 5% fiber	17,3% protein (fish, soybean, wheat, corn) 5% fat (fish, soy oil, wheat, corn, alfalfa) 56.9% carb (ground wheat and corn) 3.9% from sucrose 5-7% crude fiber
Vitamin Supplements	Only for CR monkeys	40% of RDA in Vitamin in both control and CR monkeys
Food intake measurement	Daily quantification for each animal	1 week per year