Extended Data Figure 10. dG•dT Misincorporation probabilities and correlation to WC-like excited states.

a, Explicit dGTP•dT and dG•dTTP kinetic misincorporation and base substitution probabilities (n=53) and associated errors^46-48,72-77 (see Supplementary Discussion 8) are plotted against hp-GT dG•dT ES1 (blue squares) and ES2 (green triangles). The pK_a fit of ES2 probabilities to the Henderson-Hasselbalch equation (Eqn. 4, see Methods) is shown as the green trend line. b, Red trend line shows the pK_a fit to dGTP•dT misincorporation probabilities⁴⁷ from pH 6.5-8.6 to the Henderson-Hasselbalch equation. The fit was weighted using reported experimental errors and gave a reduced χ² of 3.56. c-d, Extrapolated dG•dT^– ES2 probability (s.e. from the weighted global fit) is plotted against dGTP•dT (left) and dG•dTTP (right) misincorporation probabilities (errors as given)⁴⁷ from pH 6.5–9.5.