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. Author manuscript; available in PMC: 2016 Mar 19.
Published in final edited form as: Nature. 2015 Mar 11;519(7543):315–320. doi: 10.1038/nature14227

Figure 5. Correlation between WC-like bps and misincorporation probabilities.

Figure 5

a, dG•dT ES1 (blue square) and ES2 (green triangle) probabilities. pH-dependent dGTP•dT*/dG•dTTP* misincorporation probabilities (errors as published)47. Averaged dGTP•dT/dG•dTTP misincorporation and base substitution probabilities; error bars reflect the s.d. (Supplementary Discussion 8). ES2 fit to Henderson-Hasselbalch equation shown as green line (see Methods). A blue line connects ES1 points for visualization purposes. b, rG•rU ES1 (blue square) and ES2 (green triangle) probabilities measured at varied conditions (Supplementary Table 1), and amino acid misincorporation probabilities (red ‘X’) due to rG•rU mispairs (Zhang et al.44). c, The probabilities of dGTP•dT/dG•dTTP misincorporation (error as given) by AMV RT (Yu et al.47) versus the pKa-predicted probability (s.e.) of forming a WC-like dG•dT– mispair as a function of pH.