. 2015 Aug 24;10(8):e0132881. doi: 10.1371/journal.pone.0132881

Table 3. NAs degradation genes.

Process	Step	Gene symbol	EC No.	Definition	Gene(s) identification NO.	Isoenzyme gene(s) identification NO. ¹
Ring-cleavage	Acylation	aliA	EC:6.2.1.-	Cyclohexanecarboxylate-CoA ligase	CR3_3384	CR3_0434, 0592, 1107, 1488, 2610, 3988
	Vinylation	aliB	EC:1.3.99.-	Cyclohexanecarboxyl-CoA dehydrogenase	/	CR3_0081, 0375, 0581, 0714, 0875, 0876, 1202, 3066, 3611
	Alcoholization	- ²	EC:4.2.1.-	Cyclohex-1-ene-1-carboxyl-CoA hydratase	/	CR3_0877, 0878
	Aldehyde	badH	EC:1.1.1.-	2-hydroxycyclohexanecarboxyl-CoA dehydrogenase	/	CR3_0933, 2047, 3549, 4177
	Ortho-ring cleavage	badI	EC:3.1.2.-	2-ketocyclohexanecarboxyl-CoA hydrolase	/	CR3_0381, 0556, 1032, 1466, 1527, 1604, 2439, 3536, 3576
Acyclic-open chain degradation	Vinylation	DCAA	EC:1.3.99.-	Acyl-CoA dehydrogenase	CR3_3611	CR3_0081, 0375, 0581, 0714, 0875, 0876, 1202, 3066
	Alcoholization	echA	EC:4.2.1.17	Enoyl-CoA hydratase	CR3_0053, 1061, 2437	/
		- ²	EC:4.2.1.-	-	CR3_0877 CR3_0878	/
	Aldehyde	fadN ³	EC:1.1.1.35	3-hydroxyacyl-CoA dehydrogenase	CR3_0378, 1482, 3304	/
		fadB	EC:1.1.1.157	3-hydroxybutyryl-CoA dehydrogenase	CR3_0216, 0899	/
	Deacetylation	fadA	EC:2.3.1.16	Acetyl-CoA acyltransferase	CR3_0379, 4448	/
		- ²	EC:2.3.1.-	-	CR3_1023, 1465, 2262	CR3_0137, 0138, 0139, 1571, 2147, 2925, 2964, 4321
	Further deacetylation	fadA	EC:2.3.1.16	Acetyl-CoA acyltransferase	CR3_0379, 4448	/
		- ²	EC:2.3.1.-	-	CR3_1023, 1465, 2262	CR3_0137, 0138, 0139, 1571, 2147, 2925, 2964, 4321
		atoB	EC:2.3.1.9	Acetyl-CoA C-acetyltransferase	CR3_0079, 1480, 1566, 1570	/
	Deacylation	putA ⁴	EC:1.2.1.3 ³	NAD-dependent aldehyde dehydrogenase ⁴	CR3_1990, 2485, 3236, 3377, 3395, 3859, 3905, 4330⁴	/

1. Isoenzyme genes were defined as a gene assigned to a same enzyme class with the same EC NO.

2. Gene symbol was unknown.

3. The fadN gene was assigned to a same enzyme class and definition as HADH gene, except KEGG ko orthology.

4. The CR3 genome did not encode an acetaldehyde dehydrogenase (EC1.2.1.10), but encoded week specific enzyme of aldehyde dehydrogenase (EC1.2.1.3).