Fig 4. Qualitative effects of ectopic Egr/TNFα and increased apoptosis in the larval imaginal wing disc.

(A) Control disc showing ectopic expression in the posterior region of wing discs using hh-GAL4 visualized by co-expression of GFP. (B) Ci staining of control discs show staining of the anterior compartment and is complementary to the region of GFP expression. (C) Caspase 3 staining revealed low levels of apoptosis in control imaginal wing discs. (D) Merged image where GFP is green, Caspase 3 staining is magenta and Ci is yellow. (E-E’) Ectopic expression of Eiger/TNFα resulted in a significant decrease in disc size and disruption to the pattern of GFP expression with punctate staining in the central wing pouch region. (F-F’) Staining of the anterior compartment with Ci reveals expression beyond the boundary and overlapping with the region of GFP expression. (G-G’) Caspase 3 staining reveals high levels of staining in the central wing pouch region and in two distinct regions extending towards to anterior portion of the disc (indicated with the red asterisks). (H-H’) Merged image (GFP is green, Caspase 3 is magenta and Ci is yellow). In all images the dotted line outlines the regions of GFP expression corresponding to the posterior region of the discs. Red boxes indicate the regions that are enlarged in E’-H’.