Fig 6. WWOX is required for elimination of Scribbled (Scrib) mutant clones.

(A-A’) Clones of cells mutant for Scrib generated in the eye using the MARCM system are positively labelled with GFP. (B-B’) Elav staining reveals absence of differentiated photoreceptors within a portion of Scrib mutant clones. (C-C’) Merged image showing GFP in green and Elav in magenta. (D-D’) GFP expressing Scrib mutant clones with decreased WWOX expression (Scrib ¹; WWOX-IR). (E-E’) Elav staining reveals absence of differentiated photoreceptors within a portion of the Scrib ¹;WWOX-IR mutant clones. (F-F’) Merged image showing GFP in green and Elav in magenta. Red boxes indicate the regions that are enlarged in D’-F’. (G) Quantification of the proportion of GFP expressing cells showed a significant increase when WWOX expression was decreased in the Scrib mutant clonal tissue compared to the Scrib mutant clones alone. (H) Quantification of total area of the eye disc containing Scrib clones with and without WWOX knockdown revealed no significant difference. (I) Clones of cells mutant for Scrib generated in the eye using the MARCM system result in a mild adult rough eye phenotype. (J) Decreased WWOX expression in the Scrib mutant clones gave a stronger phenotype with a decreased eye size, significant disruption to ommatidial patterning and the presence of necrotic lesions. (K) Quantification of the overall size of the adult eyes showed a significant decrease when WWOX levels were knocked down in Scrib mutant clones. (L) Quantification of percentage of adult fly eye showing necrotic spots of different sizes: Small (550–3000 pixels), Medium (3000–5500 pixels) or Large (>5500 pixels). Genotypes used in these experiments: Scrib ¹ = (ey-FLP1, UAS-mCD8-GFP;;tub-GAL4 FRT82B tub-GAL80/ FRT82B, Scrib ¹ ), Scrib ¹;WWOX-IR = (ey-FLP1, UAS-mCD8-GFP; UAS-WWOX-IR#2/+;tub-GAL4 FRT82B tub-GAL80/ FRT82B, Scrib ¹ ). Significance indicated by * = p<0.05 and ** = p<0.005, ns = not significant.