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. 2015 Aug 24;10(8):e0136355. doi: 10.1371/journal.pone.0136355

Table 5. Optimal and worst reference genes in four sample sets using three methods.

Sample sets Optimal reference gene Worst reference gene
geNorm NormFinder BestKeeper Aggregated result geNorm NormFinder BestKeeper Aggregated result
SYBR Green
Total OfRAN1, OfUBC2 OfUBC2 OfRAN1 OfRAN1, OfUBC2 Of18S Of18S Of18S Of18S
Cultivars OfRAN1, OfUBC2 OfUBC2 OfRAN1 OfRAN1, OfUBC2 Of18S Of18S OfIDH Of18S
Developmental stages OfACT, OfEF1α OfACT, OfEF1α OfIDH OfACT, OfEF1α Of18S Of18S Of18S Of18S
Temperature treatments OfACT, OfIDH OfACT OfRAN1 OfACT Of18S Of18S OfEF1α Of18S
EvaGreen
Total OfACT, OfUBC2 OfUBC2 OfRAN1 OfUBC2 Of18S Of18S Of18S Of18S
Cultivars OfEF1α, OfUBC2 OfUBC2 OfRAN1 OfUBC2 Of18S Of18S OfIDH Of18S
Developmental stages OfACT, OfUBC2 OfACT OfEF1α OfACT Of18S Of18S Of18S Of18S
Temperature treatments OfACT, OfEF1α OfACT OfTUB OfACT Of18S Of18S OfIDH Of18S