Table 5. Optimal and worst reference genes in four sample sets using three methods.

Sample sets	Optimal reference gene				Worst reference gene
	geNorm	NormFinder	BestKeeper	Aggregated result	geNorm	NormFinder	BestKeeper	Aggregated result
SYBR Green
Total	OfRAN1, OfUBC2	OfUBC2	OfRAN1	OfRAN1, OfUBC2	Of18S	Of18S	Of18S	Of18S
Cultivars	OfRAN1, OfUBC2	OfUBC2	OfRAN1	OfRAN1, OfUBC2	Of18S	Of18S	OfIDH	Of18S
Developmental stages	OfACT, OfEF1α	OfACT, OfEF1α	OfIDH	OfACT, OfEF1α	Of18S	Of18S	Of18S	Of18S
Temperature treatments	OfACT, OfIDH	OfACT	OfRAN1	OfACT	Of18S	Of18S	OfEF1α	Of18S
EvaGreen
Total	OfACT, OfUBC2	OfUBC2	OfRAN1	OfUBC2	Of18S	Of18S	Of18S	Of18S
Cultivars	OfEF1α, OfUBC2	OfUBC2	OfRAN1	OfUBC2	Of18S	Of18S	OfIDH	Of18S
Developmental stages	OfACT, OfUBC2	OfACT	OfEF1α	OfACT	Of18S	Of18S	Of18S	Of18S
Temperature treatments	OfACT, OfEF1α	OfACT	OfTUB	OfACT	Of18S	Of18S	OfIDH	Of18S