Figure 5.

Three-dimensional sub-cellular structure of the frozen-hydrated N. caninum cell. (a) A deconstructed isosurface model of the N. caninum tachyzoite is shown with two-dimensional projections along the beam direction of three-dimensional renderings of each region chosen to be highlighted (see Table S1). At the centre, the boundary of the cell is shown, as well as each of the regions in its corresponding location within the cell. Regions are numbered and coloured according to their listing in Table S1. A line extends from each of these regions to their cut-outs, which are numbered and bound by the three lengths of the box that circumscribes them; scale bars: 500 nm. (b) Views of the three-dimensional isosurface model of the tachyzoite along the three primary axes. The Z axis corresponds to a view along the beam direction at the 0° tilt angle. (c) Starting from the 0° tilt angle, the Z-axis view in (b) is rotated about a primary axis, each frame (I–VI) in the montage is a 60° tilt apart from the previous.