. Author manuscript; available in PMC: 2015 Aug 24.

Published in final edited form as: Environ Sci Process Impacts. 2014 Feb;16(2):341–351. doi: 10.1039/c3em00570d

Table 1.

Comparison of analytical chemistry method treatment conditions used to digest metal study powders and human in vivo skin surface conditions for the determination of allergen mass

		Treatments
Sensitizer	Protocol	Reactants^a	Thermal	Agitation
Be - soluble	Profumo et al.²⁰	0.01 M HCl	Ambient temperature (20 °C)	Ultrasonic
Ni - soluble	Zatka et al.²¹	0.1 M Citrate	Ambient temperature (20 °C)	Orbital shaker
Cr - total	NIOSH 7303	Cone. HNO₃/HCl	Hot block (95 °C)	None
All (Be, Ni, Cr)	Sweat extraction	Artificial sweat	Skin (36 °C)	None

HCl = hydrochloric acid (pH 2.0); citrate = ammonium citrate/citric acid buffer (pH 4.4); cone. HNO₃/HCl = concentrated nitric acid/hydrochloric acid (pH < 1); artificial sweat (pH 5.3) from Harvey et al.²²