Figure 4.

Higher levels of resistance evolve at antibiotic concentrations above scMIC

1. Design of the laboratory evolution experiment. Identical clonal populations of cells were evolved over 13 days with daily dilution by 225 in media with various antibiotic concentrations. The increase of the scMIC of the population at the end of the experiment was observed in the media where antibiotic concentrations were above scMIC of the initial population.
2. Laboratory evolution experiments of the reference strain confirm that increase of resistance evolves in antibiotic concentrations equal to and larger than the scMIC. Plotted is the scMIC measured after 13 days versus the concentration of cefotaxime the strains were evolved in. The error bars are the standard errors of the mean of six independently evolved populations. The gray bars correspond to the initial scMIC values ± s.e.m.

Source data are available online for this figure.