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. Author manuscript; available in PMC: 2016 Sep 24.
Published in final edited form as: Neuroscience. 2015 Jul 23;304:161–175. doi: 10.1016/j.neuroscience.2015.07.052

Figure 8. Inhibition of α6-containing nAChRs in VTA blocks AMPAR enhancement by systemic nicotine.

Figure 8

A) Experimental design. α6L9S mice were cannulated and vehicle or αCtxMII (10 pmol) was infused into the VTA. Following VTA infusion, mice were injected i.p. with saline or nicotine (0.03 mg/kg). Sixty min later, brain slices were prepared for recording. AMPA-evoked currents were elicited by locally puffing AMPA onto the cell body of the recorded neuron and recording inward cation currents in voltage clamp mode.

B) Representative AMPA-evoked currents from α6L9S mice injected/infused with the indicated drugs are shown.

C) Mean peak AMPA-evoked currents for each group shown in (B) are plotted. Mann-Whitney U-test: *p<0.05 (actual: p=0.0205), **p<0.01 (actual: p=0.003). (VEH/VEH: n=7, MII/VEH: n=7, VEH/NIC: n=8, MII/NIC: n=8)

D) Cannula location for each mouse in groups indicated in (B) is shown.