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. 2015 Jun 13;34(14):1971–1985. doi: 10.15252/embj.201490878

Figure 6.

Figure 6

FancI–FancD2 is not essential for chromatin recruitment of Rev1 and Rev7

  • A Immunodepletion of FancD2 and FancI. A dilution series of mock-depleted NPE, FancI/FancD2-depleted NPE, and FancI/FancD2-depleted NPE supplemented with recombinant wild-type xlFancD2/FancI complex were analyzed by Western blotting using FancD2 and FancI antibodies.
  • B Lesion bypass in mock-depleted and FancI/FancD2-depleted extracts and in FancI–FancD2-depleted extracts supplemented with wild-type recombinant FancI–FancD2 complex was analyzed as described in Fig2B. Accumulation of the −1 product shows that the ID-depleted egg extract was functionally depleted.
  • C–E Extracts depleted as in (B) were analyzed by ChIP using antibodies against FancI (C), Rev1 (D), and Rev7 (E). An experimental replicate of (E) is shown in Supplementary Fig S6B.

Source data are available online for this figure.