Table 3.
Comparison of media compositions for Minimum Essential Medium Alpha (AMEM) and Dulbecco’s Modified Eagle’s Medium (DMEM).
| Component | AMEM (mg/L) | DMEM (mg/L) |
|---|---|---|
| Inorganic Salts | ||
| CaCl2 | 200 | 200 |
| Fe(NO3)3 • 9H2O | — | 0.1 |
| KCl | 400 | 400 |
| MgSO4 | 97.7 | 97.7 |
| NaCl | 6800 | 6400 |
| NaHCO3 | 2200 | 3700 |
| NaH2PO4 | 121.7 | 109 |
| Amino Acids | ||
| L-Alanine | 25 | — |
| L-Arginine • HCl | 126.4 | 84 |
| L-Asparagine • H2O | 50 | — |
| L-Aspartic Acid | 30 | — |
| L-Cysteine • HCl • H2O | 100 | — |
| L-Cysteine • 2HCl | 31.2 | 62.6 |
| L-Glutamic Acid | 75 | — |
| L-Glutamine | — | 584 |
| Glycine | 50 | 30 |
| L-Histidine • HCl • H2O | 41.9 | 42 |
| L-Isoleucine | 52.5 | 105 |
| L-Leucine | 52.5 | 105 |
| L-Lysine HCl | 72.5 | 146 |
| L-Methionine | 15 | 30 |
| L-Phenylalanine | 32.5 | 66 |
| L-Proline | 40 | — |
| L-Serine | 25 | 42 |
| L-Threonine | 47.6 | 95 |
| L-Tryptophan | 10 | 16 |
| L-Tyrosine • 2Na+ • 2H2O | 51.9 | 103.8 |
| L-Valine | 46.8 | 94 |
| Vitamins | ||
| Ascorbic Acid | 50 | — |
| Biotin | 0.1 | — |
| D-Ca2+ Pantothenate | 1 | 4 |
| Choline Chloride | 1 | 4 |
| Folic Acid | 1 | 4 |
| i-Inositol | 2 | 7.2 |
| Niacinamide | — | 4 |
| Nicotinamide | 1 | — |
| Pyridoxine • HCl | 1 | 4 |
| Riboflavin | 0.1 | 0.4 |
| Thiamine • HCl | 1 | 4 |
| Vitamin B | 1.36 | — |
| Other | ||
| D-Glucose | 1000 | 4500 |
| Lipoic Acid | 0.2 | — |
| Phenol Red, Na+ | 10 | 15.9 |
| Sodium Pyruvate | 110 | 110 |