Table 1. Mean percentages (± SEM) of morulae and day 7 blastocysts and numbers of hatching blastocysts and cells in embryo cultured in groups without EGF supplementation and those cultured singly with 0, 5, 25, 50 and 100 ng/ml EGF.
| Group | No. of cleaved embryos |
No. of cleaved embryos developed to |
No. of hatching blastocysts |
Cell numbers | |
| Morula (%) | Day 7 blastocyst (%) | ||||
| Group w/o EGF | 65 | 41 (63.1 ± 6.4) | 36 (55.4 ± 2.9)a | 14 (38.9 ± 7.2) | 155.3 ± 11.2 |
| EGF 0 ng/ml | 55 | 36 (65.5 ± 5.8) | 20 (36.4 ± 7.7)b | 5 (25.0 ± 11.4) | 146.3 ± 9.3 |
| EGF 5 ng/ml | 57 | 37 (64.9 ± 6.4) | 32 (56.1 ± 6.6)a | 14 (43.8 ± 16.0) | 186.7 ± 9.1 |
| EGF 25 ng/ml | 61 | 35 (57.4 ± 6.4) | 26 (42.6 ± 6.4)a | 9 (34.6 ± 10.0) | 161.4 ± 10.2 |
| EGF 50 ng/ml | 58 | 33 (56.9 ± 6.6) | 30 (51.7 ± 6.6)a | 13 (43.3 ± 6.2) | 188.0 ± 12.7 |
| EGF 100 ng/ml | 66 | 45 (68.2 ± 5.8) | 36 (54.5 ± 6.2)a | 13 (36.1 ± 5.6) | 166.6 ± 17.1 |
a,b Within a column, different superscripts denote values that differ significantly (P ≤ 0.05). w/o, without.