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. 2015 May 11;61(4):305–316. doi: 10.1262/jrd.2015-027

Fig. 3.

Fig. 3.

Increased apoptosis and abnormal cell cycle progression of Rb1 KO GS cells. (A) Appearance of Rb1 KO GS cells. (B) Impaired proliferation of Rb1 KO GS cells (n = 3). Cells were recovered 2 weeks after AxCANCre transfection. (C) Analysis of cell cycle distribution using Hoechst 33342. Cells were analyzed 2 weeks after AxCANCre transfection. (D) Quantification of cell cycle phases by combined propidium iodide and BrdU staining. Cells were analyzed 2 weeks after AxCANCre transfection (n = 3). (E) Quantification of apoptotic cells using TUNEL staining (n = 3). At least 273 cells were counted 1 week after AxCANCre transfection. (F) Real-time PCR analysis of spermatogonia markers (n = 6). (G) Flow cytometric analysis of cell surface markers. Bar = 50 μm (A, E).