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Analysis of undifferentiated spermatogonia in Ddx4-Cre Rb1flox/– mice. (A–C) Immunohistochemical analysis of γH2AX, RAD51 or pTRP53 expression in GFRA1+ (A), CDH1+ (B) or KIT+ (C) cells in the 10-day-old Ddx4-Cre Rb1flox/– mouse testis. At least 50 tubules were counted in three testes. (D) TUNEL staining of the 10-day-old Ddx4-Cre Rb1flox/– mouse testis. At least 50 tubules were counted in three testes. Representative pictures are shown in Supplementary Fig. 3 (online only).
