Table 2. Post-thaw sperm physiology and viability parameters for the giant grouper (E. lanceolatus) with different sperm diluents (10% DMSO).

Sperm diluents	Ratio of fast motion (%)	Ratio of slow motion (%)	Time of fast motion (sec)	Time of slow motion (sec)	Lifespan (sec)	Motility (%)
ELS1	11.66 ± 1.37bc	26.36 ± 1.38cde	27.52 ± 5.29ce	115.35 ± 32.09d	721.05 ± 87.87de	38.03 ± 0.94de
ELS2	5.60 ± 1.66defg	17.38 ± 2.52e	65.32 ± 27.54de	115.33 ± 43.94d	746.57 ± 121.57de	22.98 ± 3.29fg
ELS3	12.78 ± 0.39bc	38.3183 ± 1.50b	114.63 ± 3.49bc	334.58 ± 20.63b	955.52 ± 37.314bc	51.10 ± 1.88c
EM1-2	3.89 ± 0.88fg	16.22 ± 1.87e	26.05 ± 4.82e	89.05 ± 14.66d	622.23 ± 135.37e	20.12 ± 2.74g
TS-2	9.15 ± 1.80cd	19.81 ± 3.63de	84.27 ± 18.82cd	239.17 ± 54.75c	958.48 ± 38.60bc	28.97 ± 5.35efg
MPRS	1.62 ± 0.66g	29.78 ± 8.34bcd	54.43 ± 5.94de	155.68 ± 18.49d	865.50 ± 71.85bcd	31.40 ± 8.57efg
ELRS0	8.70 ± 5.39cde	24.25 ± 9.15de	45.66 ± 9.05e	121.43 ± 12.68d	788.42 ± 67.21cde	32.95 ± 14.51defg
ELRS1	4.34 ± 1.03efg	30.38 ± 9.47bcd	30.45 ± 3.41e	115.29 ± 25.86d	656.28 ± 33.03e	34.72 ± 10.01def
ELRS2	6.77 ± 1.91def	21.40 ± 12.16de	104.45 ± 16.71c	234.27 ± 13.29c	875.32 ± 132.08bcd	28.17 ± 11.44efg
ELRS3	15.04 ± 4.95b	54.40 ± 7.29a	121.37 ± 10.84b	336.27 ± 10.65b	1039.60 ± 72.28b	69.44 ± 8.06b
ELRS4	9.60 ± 4.37cd	26.55 ± 3.61cde	133.88 ± 9.24b	232.38 ± 46.48c	860.97 ± 69.95bcd	36.15 ± 7.96def
ELRS5	8.74 ± 2.64cde	36.23 ± 1.43bc	42.07 ± 9.31e	95.73 ± 11.06d	670.40 ± 101.48e	44.97 ± 3.61cd
ELRS6	1.24 ± 0.15g	21.49 ± 2.62de	82.31 ± 9.52de	211.05 ± 24.77c	904.17 ± 43.96bcd	22.74 ± 2.73fg
Fresh sperm	30.34 ± 1.17a	50.37 ± 0.32a	450.43 ± 105.75a	1114.67 ± 155.67a	1919.96 ± 364.52a	80.71 ± 1.49a

All motion-related parameters were better for sperm in ELRS3 and ELS3 than those in other solutions, although their values were significantly lower than those of fresh sperm (n = 6, P < 0.05). Values are means ± standard deviation, and means with different letters (a, b, c, d, e, f, g) are significantly different (P < 0.05).