. 2015 May 18;61(4):333–339. doi: 10.1262/jrd.2014-087

Table 3. Post-thaw sperm physiology and viability parameters for the giant grouper (E. lanceolatus) with different DMSO concentrations in ELS3 and ELRS3.

Sperm diluents	Concentration of DMSO (%)	Ratio of fast motion (%)	Ration of slow motion (%)	Time of fast motion (sec)	Time of slow motion (sec)	Lifespan (sec)	Motility (%)
ELS3	0	0.66 ± 0.48^c	4.20 ± 1.55^c	49.73 ± 8.47^d	155.53 ± 50.03^c	356.60 ± 89.57^de	4.86 ± 1.66^c
	5	6.87 ± 3.20^bc	23.03 ± 4.70^bc	56.93 ± 23.48^d	165.83 ± 43.92^c	673.00 ± 266.38^cd	29.90 ± 3.45^c
	10	15.74 ± 6.41^b	35.64 ± 5.08^ab	200.37 ± 31.38^c	430.43 ± 132.84^b	868.67 ± 218.65^c	51.38 ± 3.25^b
	15	28.85 ± 8.99^a	39.51 ± 17.79^ab	331.67 ± 24.34^b	558.97 ± 37.34^b	1373.67 ± 238.34^b	68.37 ± 25.23^ab
	20	15.90 ± 9.45^b	39.45 ± 16.63^ab	306.10 ± 46.13^b	421.20 ± 84.03^b	1258.90 ± 119.39^b	55.35 ± 16.98^b
	30	5.75 ± 3.33^c	8.87 ± 1.50^c	202.23 ± 27.79^c	360.13 ± 52.80^b	162.67 ± 32.13^e	14.62 ± 4.17^c

ELRS3	0	3.16 ± 2.29^b	7.35 ± 1.45^d	47.23 ± 15.34^c	106.70 ± 7.07^d	293.50 ± 86.10^c	10.51 ± 3.69^c
	5	7.04 ± 3.49^b	14.86 ± 1.35^cd	91.37 ± 36.83^c	186.23 ± 55.52^d	320.57 ± 103.95^c	28.56 ± 8.12^bc
	10	20.56 ± 10.81^a	21.52 ± 10.11^c	132.00 ± 43.21^c	268.70 ± 51.52^d	774.47 ± 166.41^bc	35.42 ± 11.92^b
	15	33.43 ± 11.65^a	41.33 ± 2.68^b	327.47 ± 27.81^b	814.87 ± 31.14^b	1795.13 ± 38.26^a	74.75 ± 12.71^a
	20	5.47 ± 2.04^b	20.55 ± 1.69^c	156.23 ± 43.35^c	426.40 ± 76.69^c	1221.30 ± 532.15^b	26.02 ± 3.22^bc
	30	4.90 ± 3.55^b	17.73 ± 6.39^c	90.90 ± 10.09^c	203.23 ± 31.58^d	499.40 ± 38.34^cd	22.63 ± 9.43^bc

Fresh sperm		30.33 ± 1.07^a	50.37 ± 0.31^a	450.23 ± 105.85^a	1114.57 ± 156.67^a	1919.90 ± 367.52^a	80.70 ± 1.37^a

The highest sperm parameters were obtained using 15% DMSO in ELRS3, and relatively high average values were obtained for sperm parameters using 15% DMSO in ELS3 (n = 3, P < 0.05). Values are means ± standard deviation, and means with different letters (a, b, c, d, e) are significantly different (P < 0.05).