. 2015 May 18;61(4):333–339. doi: 10.1262/jrd.2014-087

Table 4. Post-thaw sperm physiology and viability parameters for the giant grouper (E. lanceolatus) with different concentrations of FBS in ELS3 (15% DMSO) and ELRS3 (15% DMSO).

Sperm diluents	Concentration of FBS (%)	Ratio of fast motion (%)	Ratio of slow motion (%)	Time of fast motion (sec)	Time of slow motion (sec)	Lifespan (sec)	Motility (%)
ELS3	0	1.94 ± 0.99^c	20.20 ± 3.49^b	144.20 ± 75.37^b	227.13 ± 53.92^c	663.53 ± 166.47^c	22.14 ± 4.44^c
	5	3.78 ± 1.67^bc	22.75 ± 6.17^b	189.13 ± 78.02^b	315.17 ± 13.71^bc	823.47 ± 196.10^c	26.53 ± 7.83^bc
	10	29.68 ± 4.17^a	41.49 ± 5.39^a	309.63 ± 31.60^ab	432.70 ± 51.75^b	1319.83 ± 71.91^b	71.16 ± 1.34^a
	15	10.40 ± 5.23^b	21.83 ± 5.87^b	310.20 ± 153.45^ab	305.10 ± 99.78^bc	825.30 ± 42.80^c	32.22 ± 5.25^bc
	20	10.57 ± 3.24^b	29.03 ± 8.07^b	126.47 ± 22.76^b	168.87 ± 47.90^c	894.67 ± 106.06^c	39.60 ± 11.29^b

ELRS3	0	4.29 ± 2.63^c	18.99 ± 5.90^b	66.97 ± 30.92^c	227.17 ± 61.07^b	476.93 ± 58.77^c	23.28 ± 3.89^d
	5	6.14 ± 3.15^c	25.85 ± 7.46^b	133.53 ± 54.56^bc	270.50 ± 93.71^b	525.00 ± 189.90^c	31.99 ± 9.97^c
	10	20.59 ± 2.47^b	43.09 ± 6.51^a	239.83 ± 47.05^b	354.70 ± 58.07^b	1080.67 ± 53.01^b	63.68 ± 4.16^b
	15	7.25 ± 3.35^c	18.95 ± 1.89^b	132.77 ± 33.97^bc	245.43 ± 36.18^b	573.77 ± 85.64^c	26.20 ± 4.79^d
	20	10.35 ± 3.61^c	21.74 ± 6.25^b	148.47 ± 52.90^bc	327.90 ± 73.91^b	511.27 ± 13.57^c	32.09 ± 4.63^c

Fresh sperm		30.33 ± 1.07^a	50.37 ± 0.31^a	450.23 ± 105.85^a	1114.57 ± 156.67^a	1919.90 ± 367.52^a	80.70 ± 1.37^a

The highest sperm parameters were obtained for 10% FBS in ELS3 and ELRS3 (n = 3, P < 0.05). Values are means ± standard deviation, and means with different letters (a, b, c, d) are significant.