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. 2015 May 22;61(4):341–350. doi: 10.1262/jrd.2014-173

Table 1. Sequences of primer pairs for detection of Gfpt, Ogt and Oga mRNAs.

Gene Sequence Remarks
Gfpt Forward ACTCGCTGTTTCCTGTGCC Rate-limiting enzyme for HBP
Reverse TCCAGTGCCTTAACTTTTCCC

Ogt Forward AGCGGGACTCAATTACCCTTT Add O-GlcNAc to proteins
Reverse ATTGTCTGGCTCTTGTCTCCA

Oga Forward AAAAGCATGATGGCTTGCCT Remove O-GlcNAc from proteins
Reverse CCACCCACCAGGTGAAGTTAA

Ubca Forward AAAAGCATGATGGCTTGCCT Reference gene
Reverse CCACCCACCAGGTGAAGTTAA

a Kuijk et al., 2007.