Table 1. Sequences of primer pairs for detection of Gfpt, Ogt and Oga mRNAs.
Gene | Sequence | Remarks | |
Gfpt | Forward | ACTCGCTGTTTCCTGTGCC | Rate-limiting enzyme for HBP |
Reverse | TCCAGTGCCTTAACTTTTCCC | ||
Ogt | Forward | AGCGGGACTCAATTACCCTTT | Add O-GlcNAc to proteins |
Reverse | ATTGTCTGGCTCTTGTCTCCA | ||
Oga | Forward | AAAAGCATGATGGCTTGCCT | Remove O-GlcNAc from proteins |
Reverse | CCACCCACCAGGTGAAGTTAA | ||
Ubca | Forward | AAAAGCATGATGGCTTGCCT | Reference gene |
Reverse | CCACCCACCAGGTGAAGTTAA |
a Kuijk et al., 2007.