Fig. 6.

Impact of the inhibition of either ERK or JNK on H2AX phosphorylation. a HaCaT cells were irradiated at 2 Gy with or without an ERK-specific inhibitor and then co-stained with DAPI and anti-γH2AX fluorescent antibodies. The γH2AX-specific signal was quantified at the single-cell level. b Similar to A with the JNK-specific inhibitor. c γH2AX content was also analyzed by Western blotting with or without inhibitors and with or without irradiation. Two independent biological replicates were performed for c