Figure 2.

L. major–specific CD4 T cells are resident in immune skin. (A) Flank skin was collected from naive or immune mice at various time points after L. major infection, and IFN-γ–producing CD4⁺ T cells detected as in Fig. 1. Data shown represent the mean (±SEM) percent IFN-γ–producing CD4⁺ T cells detected by flow cytometry (n = 3–4 mice per time point). (B) Flank skin from immune mice (13–14 wk after infection) was grafted onto naive mice, and at various times the grafts were harvested and IFN-γ–producing CD4⁺ T cells were detected as in Fig. 1. Quantification of L. major–specific cells in skin grafts before grafting (time 0) and 1, 2, or 4 wk later is shown. Data are combined from two separate experiments and each dot represents one mouse. *, P < 0.05.