Skip to main content
. 2015 Aug 24;212(9):1433–1448. doi: 10.1084/jem.20141555

Figure 3.

Figure 3.

Macrophage-specific FLT1 knockout inhibits human breast tumor cell distal metastasis. (a) Experimental metastasis assay of 4173 cells in nude mice with macrophage-specific Csf1r-iCre–induced Flt1 knockout and Cre− littermate controls. (b and c) Representative H&E-stained lung section of lung metastasis in the indicated mice. (d–f) Stereological quantification of the distal metastasis efficiency of 4173 cells in macrophage-specific Flt1 knockout and littermate control. Metastasis quantification was the same as in Fig. 1. Data show mean + SEM. n ≥ 5; *, P < 0.05; **, P < 0.01 by Student’s t test. (g) Representative micrographs showing colocalization of immunofluorescently stained FLT1 with macrophage markers using anti-CD68 (top) and CD163 (bottom) antibodies in patient-derived breast tumor samples. (h) Representative FLT1 (top) and CD68 (bottom) immunohistochemistry staining–labeled tumor stroma in breast cancer metastasis samples. Bars: (b and c) 1 mm; (g) 20 µm; (h) 100 µm. (i) Number of cases of FLT1-positive (+) and FLT1-negative (-) macrophage-like stroma in primary and metastatic breast cancer samples. P is calculated with Fisher’s exact test.